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bluescript ii k/s− factor viii cloning vector  (Agilent technologies)
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Agilent technologies bluescript ii k/s− factor viii cloning vector
Thrombogram for WT (solid line), 336(P4-P3′)372 (dashed line), 336(P4-P3′)740 (dotted line), 336(P4-P3′)562 (double line), and R336Q (×) <t>FVIII</t> variants. Reactions were run using 1 nm factor <t>VIII,</t> 0.15 pm recombinant tissue factor, and 4 μm phospholipid and thrombin generation in plasma was determined through calibrated automated thrombography as described under “Experimental Procedures.” Experiments were performed at least three times with mean ± S.D. within 20%.
Bluescript Ii K/S− Factor Viii Cloning Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bluescript+ii+k/pmc03346119-158-4-13
Average 90 stars, based on 1 article reviews
bluescript ii k/s− factor viii cloning vector - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
bluescript ii k/s factor viii cloning vector  (Agilent technologies)
90
Agilent technologies bluescript ii k/s factor viii cloning vector
Thrombogram for WT (solid line), 336(P4-P3′)372 (dashed line), 336(P4-P3′)740 (dotted line), 336(P4-P3′)562 (double line), and R336Q (×) <t>FVIII</t> variants. Reactions were run using 1 nm factor <t>VIII,</t> 0.15 pm recombinant tissue factor, and 4 μm phospholipid and thrombin generation in plasma was determined through calibrated automated thrombography as described under “Experimental Procedures.” Experiments were performed at least three times with mean ± S.D. within 20%.
Bluescript Ii K/S Factor Viii Cloning Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bluescript+ii+k/10__1074_slash_jbc__m111__333948-53-4-13
Average 90 stars, based on 1 article reviews
bluescript ii k/s factor viii cloning vector - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
bluescript ii k(2) vector  (Agilent technologies)
90
Agilent technologies bluescript ii k(2) vector
Thrombogram for WT (solid line), 336(P4-P3′)372 (dashed line), 336(P4-P3′)740 (dotted line), 336(P4-P3′)562 (double line), and R336Q (×) <t>FVIII</t> variants. Reactions were run using 1 nm factor <t>VIII,</t> 0.15 pm recombinant tissue factor, and 4 μm phospholipid and thrombin generation in plasma was determined through calibrated automated thrombography as described under “Experimental Procedures.” Experiments were performed at least three times with mean ± S.D. within 20%.
Bluescript Ii K(2) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bluescript+ii+k/pm10094865-66-7-11
Average 90 stars, based on 1 article reviews
bluescript ii k(2) vector - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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Thrombogram for WT (solid line), 336(P4-P3′)372 (dashed line), 336(P4-P3′)740 (dotted line), 336(P4-P3′)562 (double line), and R336Q (×) FVIII variants. Reactions were run using 1 nm factor VIII, 0.15 pm recombinant tissue factor, and 4 μm phospholipid and thrombin generation in plasma was determined through calibrated automated thrombography as described under “Experimental Procedures.” Experiments were performed at least three times with mean ± S.D. within 20%.

Journal: The Journal of Biological Chemistry

Article Title: Sequences Flanking Arg 336 in Factor VIIIa Modulate Factor Xa-catalyzed Cleavage Rates at this Site and Cofactor Function *

doi: 10.1074/jbc.M111.333948

Figure Lengend Snippet: Thrombogram for WT (solid line), 336(P4-P3′)372 (dashed line), 336(P4-P3′)740 (dotted line), 336(P4-P3′)562 (double line), and R336Q (×) FVIII variants. Reactions were run using 1 nm factor VIII, 0.15 pm recombinant tissue factor, and 4 μm phospholipid and thrombin generation in plasma was determined through calibrated automated thrombography as described under “Experimental Procedures.” Experiments were performed at least three times with mean ± S.D. within 20%.

Article Snippet: Mutants were introduced into Bluescript II K/S− factor VIII cloning vector using the Stratagene QuikChange Site-directed Mutagenesis Kit and the mutated factor VIII coding region was ligated into the B-domainless factor VIII expression vector at XhoI/NotI restriction sites as described previously ( 20 ).

Techniques: Recombinant